U-2012: An improved Lowry protein assay, insensitive to sample color, offering reagent stability and enhanced sensitivity
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چکیده
منابع مشابه
U-2012: An improved Lowry protein assay, insensitive to sample color, offering reagent stability and enhanced sensitivity.
Traditional colorimetric protein assays such as Biuret, Lowry, and modified Lowry (U-1988) are unsuitable for colored biological samples. Here we describe an improved Lowry protein assay (U-2012), which utilizes stable reagents and offers enhanced sensitivity over the U-1988 assay. U-2012 circumvents interference from colored pigments and other substances (for example sugars) bound to perchlori...
متن کاملAn improved Lowry protein assay, insensitive to sample color, offering reagent stability and enhanced sensitivity
www.BioTechniques.com 159 Vol. 52 | No. 3 | 2012 Quantitative measures of physiological traits such as enzyme activity are often expressed as units of activity per milligram protein. Although numerous assays have been developed to measure protein content, including the colorimetric assays of Amido Black (1), Biuret (2), Bicinchoninic Acid (3) and Coomassie Blue (4,5), the Lowry assay (6) or its...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2012
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000113818